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	Provedor de dados Genet. Mol. Biol. (2) Autor Machado,Maria de Fátima P.S. (2) Carvalho,Vanda Marilza de (1) Conte,Hélio (1) Lapenta,Ana Sílvia (1) Marques,Rosimar Maria (1) Ruvolo-Takasusuki,Maria Claudia C. (1) Mais... Palavra-chave Esterase (1) Esterase classification (1) Genetic variability (1) Inhibition test (1) Isozymes (1) Moth (1) Ops (1) Peroba-rosa (1) Sugarcane borer (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2003 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Esterase-3 polymorphism in the sugarcane borer Diatraea saccharalis (Lepidoptera, Pyralidae) Genet. Mol. Biol. Ruvolo-Takasusuki,Maria Claudia C.; Machado,Maria de Fátima P.S.; Conte,Hélio. The migration rate of esterases and their substrate specificity for 4-methylumbelliferyl esters (acetate, propionate, and butyrate) and alpha- and beta-naphthyl esters were analyzed in Diatraea saccharalis by starch gel electrophoresis. Substrate preference of esterases was observed with Est-2 and Est-8 isozymes showing substrate specificity for 4-methylumbelliferyl esters and Est-4 isozyme showing specificity for 4-methylumbelliferyl butyrate and alpha-naphthyl butyrate. Allele variation was detected at the Est-3 locus. Two alleles, Est-3F and Est-3S, were identified in pupae with fluorogenic and ester-naphthyl substrates. Chi-square analysis showed no differences between the observed genotypic frequencies and those expected on the basis of Hardy-Weinberg... Tipo: Info:eu-repo/semantics/article Palavras-chave: Esterase; Genetic variability; Isozymes; Moth; Sugarcane borer. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100012 Functional classification of esterases from leaves of Aspidosperma polyneuron M. Arg. (Apocynaceae) Genet. Mol. Biol. Carvalho,Vanda Marilza de; Marques,Rosimar Maria; Lapenta,Ana Sílvia; Machado,Maria de Fátima P.S.. Polyacrylamide gel electrophoresis system (PAGE) and inhibition tests for biochemical characterization of alpha- and beta-esterases were used to obtain a functional classification of esterases fromAspidosperma polyneuron. The characterization of alpha- and beta-esterases from young leaves of A. polyneuron by the PAGE system showed fourteen esterase isozymes. The differential staining pattern showed that Est-2 isozyme hydrolyzes beta-naphthyl acetate; Est-6, Est-7 and Est-8 isozymes hydrolyze alpha-naphthyl acetate, and Est-1, Est-3, Est-4, Est-5, Est-9, Est-10, Est-11, Est-12, Est-13, and Est-14 isozymes hydrolyze both alpha- and b-naphthyl acetate. Inhibition pattern of a- and beta-esterases showed that Folidol is a more potent inhibitor that Malathion,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Esterase classification; Peroba-rosa; Ops; Inhibition test. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200013 Registros recuperados: 2 Primeira ... 1 ... Última

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